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			水稻cFBA和菠菜cpTPI串联基因在鱼腥藻7120中的表达及其对光合作用的影响-中国期刊全文数据库</title>
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<td><span class='datatitle'><font color='red'>水稻cFBA和菠菜cpTPI串联基因在鱼腥藻7120中的表达及其对光合作用的影响</font></span><br>&nbsp;&nbsp<IMG SRC='/KNS50/images/logo/cajviewer16.gif' WIDTH='16' HEIGHT='16' BORDER=0> 推荐 <a href='/KNS50/download.aspx?filename=VZVYvsiYHtWSRRmex4GNWFkS5ZTM4BjM3FkYEhFV0JVSMRzS2EnVZpUcTN3Y0FXbmJneKh2ZylmSrR1dKJkaiRUb2Z2c5kkavYXdZNXO0o1Lhd3QURTRpVlQIhFa4FkRvFXWPpXW0RmcwknYoR3UvRjNpZENJlEc&tablename=CJFD2006' target='_blank'><b>CAJ下载</b></a> <IMG SRC='/KNS50/images/logo/caj.gif' BORDER=0> 
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【英文篇名】</td>
<td width="" style='word-break:break-all'>
<span class='datatitle'>Expression tandem genes of rice cFBA and spinach cpTPI in Anabaena sp. PCC 7120 and explore its regulation in photosynthesis</span></td>
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【作者】</td>
<td width="" style='word-break:break-all'>
<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e4%bd%9c%e8%80%85&skey=%e9%99%88%e6%99%93', 'knet','status=yes,scrollbars=yes,width=375,height=250')">陈晓</a>;
<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e4%bd%9c%e8%80%85&skey=%e8%b0%ad%e7%8e%ae', 'knet','status=yes,scrollbars=yes,width=375,height=250')">谭玮</a>;
<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e4%bd%9c%e8%80%85&skey=%e5%86%af%e7%87%95', 'knet','status=yes,scrollbars=yes,width=375,height=250')">冯燕</a>;
<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e4%bd%9c%e8%80%85&skey=%e7%8e%8b%e5%85%a8%e5%96%9c', 'knet','status=yes,scrollbars=yes,width=375,height=250')">王全喜</a>;
<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e4%bd%9c%e8%80%85&skey=%e6%96%bd%e5%ae%9a%e5%9f%ba', 'knet','status=yes,scrollbars=yes,width=375,height=250')">施定基</a>;</td>
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【英文作者】</td>
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<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e8%8b%b1%e6%96%87%e4%bd%9c%e8%80%85&skey=CHEN+Xiao+TAN+Wei+FENG+Yan+WANG+Quan-xi+SHI+Ding-ji+%ef%bc%88College+of+Life+and+Environment+Sciences','knet','status=yes,scrollbars=yes,width=375,height=250')">CHEN Xiao TAN Wei FENG Yan WANG Quan-xi SHI Ding-ji （College of Life and Environment Sciences</a>;
<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e8%8b%b1%e6%96%87%e4%bd%9c%e8%80%85&skey=Shanghai+Normal+University','knet','status=yes,scrollbars=yes,width=375,height=250')">Shanghai Normal University</a>;
<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e8%8b%b1%e6%96%87%e4%bd%9c%e8%80%85&skey=Shanghai+200234','knet','status=yes,scrollbars=yes,width=375,height=250')">Shanghai 200234</a>;
<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e8%8b%b1%e6%96%87%e4%bd%9c%e8%80%85&skey=China%ef%bc%89','knet','status=yes,scrollbars=yes,width=375,height=250')">China）</a>;</td>
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【作者单位】</td>
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<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e6%9c%ba%e6%9e%84&skey=%e4%b8%8a%e6%b5%b7%e5%b8%88%e8%8c%83%e5%a4%a7%e5%ad%a6%e7%94%9f%e5%91%bd%e4%b8%8e%e7%8e%af%e5%a2%83%e7%a7%91%e5%ad%a6%e5%ad%a6%e9%99%a2', 'knet','status=yes,scrollbars=yes,width=375,height=250')">上海师范大学生命与环境科学学院</a>;</td>
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【刊名】</td>
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<a target=_blank href='/KNS50/Navi/Bridge.aspx?LinkType=BaseLink&DBCode=cjfd&TableName=cjfdbaseinfo&Field=BaseID&Value=SHDZ&NaviLink=%e4%b8%8a%e6%b5%b7%e5%b8%88%e8%8c%83%e5%a4%a7%e5%ad%a6%e5%ad%a6%e6%8a%a5(%e8%87%aa%e7%84%b6%e7%a7%91%e5%ad%a6%e7%89%88)'>上海师范大学学报(自然科学版)</a>
, <a target=_blank href='/KNS50/Navi/Bridge.aspx?LinkType=BaseLink&DBCode=cjfd&TableName=cjfdbaseinfo&Field=BaseID&Value=SHDZ&NaviLink=%e4%b8%8a%e6%b5%b7%e5%b8%88%e8%8c%83%e5%a4%a7%e5%ad%a6%e5%ad%a6%e6%8a%a5(%e8%87%aa%e7%84%b6%e7%a7%91%e5%ad%a6%e7%89%88)'>Journal of Shanghai Normal University(Natural Sciences)</a>, <a href='mailto:SHDZ@chinajournal.net.cn'>编辑部邮箱</a> 
<a target=_blank href='/KNS50/Navi/Bridge.aspx?LinkType=IssueLink&DBCode=cjfd&TableName=cjfdyearinfo&Field=BaseID*year*issue&Value=SHDZ*2006*04&NaviLink=%e4%b8%8a%e6%b5%b7%e5%b8%88%e8%8c%83%e5%a4%a7%e5%ad%a6%e5%ad%a6%e6%8a%a5(%e8%87%aa%e7%84%b6%e7%a7%91%e5%ad%a6%e7%89%88)'>2006年 04期</a>&nbsp;&nbsp;<br>期刊荣誉：ASPT来源刊&nbsp;&nbsp;CJFD收录刊</td>
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【关键词】</td>
<td width="" style='word-break:break-all'>
<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e4%b8%ad%e6%96%87%e5%85%b3%e9%94%ae%e8%af%8d&skey=%e4%b8%99%e7%b3%96%e7%a3%b7%e9%85%b8%e5%bc%82%e6%9e%84%e9%85%b6%ef%bc%88TPI%ef%bc%89','knet','status=yes,scrollbars=yes,width=375,height=250')">丙糖磷酸异构酶（TPI）</a>;
<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e4%b8%ad%e6%96%87%e5%85%b3%e9%94%ae%e8%af%8d&skey=%e6%9e%9c%e7%b3%96-1','knet','status=yes,scrollbars=yes,width=375,height=250')">果糖-1</a>;
<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e4%b8%ad%e6%96%87%e5%85%b3%e9%94%ae%e8%af%8d&skey=6-%e4%ba%8c%e7%a3%b7%e9%85%b8%e9%86%9b%e7%bc%a9%e9%85%b6%ef%bc%88FBA%ef%bc%89','knet','status=yes,scrollbars=yes,width=375,height=250')">6-二磷酸醛缩酶（FBA）</a>;
<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e4%b8%ad%e6%96%87%e5%85%b3%e9%94%ae%e8%af%8d&skey=%e8%bd%ac%e5%9f%ba%e5%9b%a0','knet','status=yes,scrollbars=yes,width=375,height=250')">转基因</a>;
<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e4%b8%ad%e6%96%87%e5%85%b3%e9%94%ae%e8%af%8d&skey=%e5%85%89%e5%90%88%e4%bd%9c%e7%94%a8%e8%b0%83%e6%8e%a7','knet','status=yes,scrollbars=yes,width=375,height=250')">光合作用调控</a>;</td>
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<td width="132" style='word-break:break-all'>
【英文关键词】</td>
<td width="" style='word-break:break-all'>
<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e8%8b%b1%e6%96%87%e5%85%b3%e9%94%ae%e8%af%8d&skey=Triosephosphate+isomerase','knet','status=yes,scrollbars=yes,width=375,height=250')">Triosephosphate isomerase</a>;
<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e8%8b%b1%e6%96%87%e5%85%b3%e9%94%ae%e8%af%8d&skey=Fructose+1','knet','status=yes,scrollbars=yes,width=375,height=250')">Fructose 1</a>;
<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e8%8b%b1%e6%96%87%e5%85%b3%e9%94%ae%e8%af%8d&skey=6-bisphosphate+aldolase','knet','status=yes,scrollbars=yes,width=375,height=250')">6-bisphosphate aldolase</a>;
<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e8%8b%b1%e6%96%87%e5%85%b3%e9%94%ae%e8%af%8d&skey=Transgene','knet','status=yes,scrollbars=yes,width=375,height=250')">Transgene</a>;
<a style='cursor:hand;color:blue;' onClick="TPI_openwindow('/KNS50/knowledgenet.aspx?sdb=CJFD&sfield=%e8%8b%b1%e6%96%87%e5%85%b3%e9%94%ae%e8%af%8d&skey=Photosynthetic+regulation','knet','status=yes,scrollbars=yes,width=375,height=250')">Photosynthetic regulation</a>;</td>
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【摘要】</td>
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利用转基因技术,将水稻cFBA和菠菜cpTPI串联基因转入到鱼腥藻7120中进行表达.结果发现:与野生藻相比,转基因藻蛋白粗提液中两个酶的比活力提高了33.3%;转基因鱼腥藻的生长明显优于野生型鱼腥藻,当在培养基中添加适量NaHCO_3时,这一优势更加明显;转基因鱼腥藻的净光合速率和真实光合速率都有明显提高,光饱和点和光补偿点也有所提高.以上这些结果表明,在鱼腥藻7120中特异的提高"非限速酶"——FBA和TPI的水平,能在一定程度上提高转基因鱼腥藻的光合作用速率和生长速度.</td>
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【英文摘要】</td>
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The tandem genes of rice cFBA and spinach cpTPI were transferred into filamentous,heterocystour cyanobacterium Anabaena sp.PCC 7120 and expressed.It was found that the transgenic cells showed a 31.2% higher in enzyme activity and grew much faster than the wild,especially in the presence of moderate NaHCO3.At the same time,net and true photosynthetic rates of transgenic cells were obviously advanced,and the light compensation and saturation points of transgenic ceils were higher than that of the wild cells.A...</td>
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【基金】</td>
<td width="" style='word-break:break-all'>
上海市教委重点科研项目(05ZZ15)</td>
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【DOI】</td>
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CNKI:SUN:SHDZ.0.2006-04-018</td>
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